Effect of LLLT on h_BMMSCs on odontoblast like cells
Inverted Contrast Light Microscope and RT- PCR Assessment
This study was designed to evaluate the effect of LLLT on odontoblast-like cells derived from human bone marrow mesenchymal stem cells.40 fasks of odontoblast-like cells were obtained from 10 samples of h-BMMSCs and divided into 2 groups; control group (A) and diode laser (GaAlAs) irradiated group (B).
Each group was further subdivided into 2 subgroups, where subgroups A1, B1 and A2, B2 were examined after 3 and 14 days after odontoblastic differentiation respectively.
Each group was examined by inverted phase contrast light microscope to examine cell count and viability as well as alizarin red stained mineralized matrix.RT-PCR was performed for each subgroup to detect, the amount of gene expression of DMP1 and ALP.
The data of cell count and RT-PCR were statistically analyzed.
Conclusion: LLLT enhanced DMP1 and ALP gene expression, cell proliferation and matrix mineralization in odontoblast-like cells derived from h-BMMSCs.
DINA MOHAMED MOHAMED EL-SAID MOHAMED HASSOUNA.
Lecturer Assistant - Faculty of Dentistry-Fayoum University.
Phd in Oral biology- Faculty of Dentistry-Ain Shams University.
Master in Oral biology - Faculty of Oral and Dental Medecine - Cairo University.
Bsc.of Oral & Dental Medecine-Cairo University